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KMID : 1188320160100050773
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Gut and Liver
2016 Volume.10 No. 5 p.773 ~ p.780
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Field Cancerization in Sporadic Colon Cancer
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Park Soo-Kyung
Song Chang-Seok
Yang Hyo-Joon
Jung Yoon-Suk
Koo Dong-Hoe
Kim Kyung-Eun
Jeong Kyung-Uk
Kim Hung-Dai
Chun Ho-Kyung
Park Dong-Il
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Abstract
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Background/Aims:Aberrant DNA methylation has a specific role in field cancerization. Certain molecular markers, including secreted frizzled-related protein 2 (SFRP2), tissue factor pathway inhibitor 2 (TFPI2), N-Myc downstream-regulated gene 4 (NDRG4) and bone morphogenic protein 3 (BMP3), have previously been shown to be hypermethylated in colorectal cancer (CRC). We aim to examine field cancerization in CRC based on the presence of aberrant DNA methylation in normal-appearing tissue from CRC patients.
Methods:We investigated promoter methylation in 34 CRC patients and five individuals with normal colonoscopy results. CRC patients were divided into three tissue groups: tumor tissue, adjacent and nonadjacent normal-appearing tissue. The methylation status (positive: methylation level >20%) of SFRP2, TFPI2, NDRG4, and BMP3 promoters was investigated using methylation-specific PCR.
Results:The methylation frequencies of the SFRP2, TFPI2, NDRG4 and BMP3 promoters in tumor/adjacent/nonadjacent normal-appearing tissue were 79.4%/63.0%/70.4%, 82.4%/53.6%/60.7%, 76.5%/61.5%/69.2%, 41.2%/35.7%/50.0%, respectively. The methylation levels of the SFRP,TFPI2, NDRG4 and BMP3 promoters in tumor tissues were significantly higher than those in normal-appearing tissue (SFRP2, p=0.013; TFPI2, p<0.001; NDRG4, p=0.003; BMP3, p=0.001). No significant correlation was observed between the methylation levels of the promoters and the clinicopathological variables.
Conclusions:The field effect is present in CRC and affects both the adjacent and nonadjacent normal-appearing mucosa.
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KEYWORD
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Colorectal neoplasms, DNA methylation, Field effect, Epigenomics
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